Antinociceptive Effects of Methanol Extract of Callophyllum inophyllum: Possible Roles of the Opioidergic, Adrenergic and Cholinergic Pathways
Keywords:
Callophyllum inophyllum, Antinociceptive, Opioid, Adrenergic, CholinergicAbstract
The antinociceptive activities of the methanol extract of Callophyllum inophyllum (CI) leaves were investigated using thermal and chemical tests of nociception: tail flick, the hot plate, acetic acid-induced writhing and the formalin-induced paw licking test. In the thermal tests, the extract delivered per oral (p.o) dose dependently resulted in the prolongation of both the hot plate and the tail flick latencies at all doses. The extract dose dependently reduced the number of abdominal constrictions in the acetic acid-induced writhing test and also considerably reduced the licking times in both phases of the formalin-induced paw licking test. While naloxone had no effect on the antinociceptive activity of the extract, both atropine and prazosin produced an inhibition of its antinociception as revealed by significant reductions in the thermal latencies compared with the control. It may therefore be concluded from this study that Chlorophyllum inophyllum antinociception is mediated via the activation of the cholinergic and alpha adrenergic pathways .